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The colour change of redprot ® solution from light pink to deep blue, which is induced by protein-dye interaction, referes to a massive change in the spectrum near 600 nm wave length.
Spectral changes of the redprot® staining fluid
1. Staining of complex mitures of proteins aeromedi
Change in absorption of redprot® caused by interaction with complex protein mixture in extracts of different pollen
2. Staining of proteins with redprot® in cells : spectra of noumerous pollen measured in situ
Figure 1: Artemisia officinalis stained with redprot®
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Figure 2: Betula vericosa stained with redprot®
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Figure 3: Lolium spec. stained with redprot®
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Figure 4: Phleum spec. stained with redprot®
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The redprot® solution contains a dye-metal ion complex:

  • Pyrogallol red (PR) + Molybdate (Mo) = dye-metal complex
  • interaction with Protein = PR-Mo-Protein complex
  • protein-selective stain: electrostatic interrelations with more than one residue
  • primarily via basic amino acids - minor contribution of aliphatic residues
  • protein interaction induces spectral changes around 600 nm
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Figure: Spectral effect on redprot® dilution by aqua dest. aeromedi
Figure: There is no effect of buffer salts visible in the spectrum at 600 nm wave length aeromedi
Biochemical application of redprot® compared to Coomassie Brilliant Blue
Application redprot®
Coomassie Blue
Protein Assay
Detection range:
10 µg to 16mg/ml 5 µg to 2 mg/ml
Membrane Staining
Detection limit

background


destaining
10 ng per spot
on NC and PVDF
free
poor interference with agents

mild
with water

100 ng per band
on PVDF
Permanent high
destaining

longlasting
with acidic alcohol solutions
Gel Staining Possible, less sensitive: 1µg/lane Highly sensitive 30 ng
Protein precipitation quantitaive and non-denaturating:
min. concentration1µg/ml
Not possible
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